A custom upright 2-photon microscope with a single laser line for 2-photon excited fluorescence imaging and second harmonic generation imaging.

Available techniques:

• 2-photon excited florescence imaing
• second harmonic generation (SHG) imaging

Objectives:

• C-Apochromat 40x/1.2 water, FWD 0.28 mm, CG 0.13-0.2
  [FWD = free working distance, CG = cover glass]

Fluorescence excitation sources:

Coherent Axon 780 nm fs-laser

Emission filters:

• Detector 1: 387/11
• Beamsplitter: 495 LP
• Detector 2: 642/80
  

Detectors and cameras:

2x Silicon photomultiplier (Hamamatsu)

Software:

• ScanImage

Other features:

• Manual stage
• Piezo focus drive

Usage fees* [SGD/hour]	NTU	Academia	Industry
	30	66	105.6
Location	ABIF, SCELSE, B2 (SBS-B2n-27P.4)
Safety Notice	Requires N3 laser license (N2/04107/0169)
Contact	abif@e.ntu.edu.sg; nobic.facilities@e.ntu.edu.sg

* Long-term discounts of 75% and 95% apply after 8 and 24 hours of continuous usage, respectively.

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