A custom upright 2-photon microscope with two laser lines, either of which can be used for fast 2-photon excited fluorescence imaging or for optogenetic stimulation.
Available techniques:
- fast 2-photon excited florescence imaging (using a resonant scanner)
- 2-photon optogenetic stimulation
- second harmonic generation (SHG) imaging
Objectives:
- W Plan-Apochromat 20x/1.0 water, FWD 1.8 mm, CG 0 mm
[FWD = free working distance, CG = cover glass]
Fluorescence excitation sources:
SPARK Alcor Duo (920 nm and 1064 nm) fs-laser
Emission filters:
- Detector 1: 520/70
- Detector 2: 593/46
Detectors and cameras:
Software:
- ScanImage
Other features:
- Resonant scanner for fast imaging
- Galvano scanner for optogentic stimulation
- Piezo focus drive
- Manual stage
| Usage fees [SGD/hour] | LKCMed | NTU | Academia | Industry |
| 10* | 35 | 66 | 105.6 | |
| Location | EMB 07-07-01, Imaging room (apply for card access) | |||
| Safety Notice | Requires N3 laser license | |||
| Contact | nobic.facilities@e.ntu.edu.sg | |||
*Reduced rates apply:
- off-peak hours (weekends, public holidays and 18.00 - 8.30 on weekdays): 70% of the rate stated in the table
- long-term:
- 30% of the prevailing rate applicable after 10 hours of booking/usage
- 30% of the prevailing rate (that is already the discounted rate in this case) applicable after 24 hours of booking/usage.