An upright 2-photon microscope equipped with a broadly tunable fs laser, resonant scanner and a module for fluorescence lifetime imaging.
Available techniques:
- fast 2-photon excited florescence imaging (using a resonant scanner)
- high-quality 2-photon excited florescence imaging (using a galvano scanner)
- 2-photon excited fluorescence lifetime imaging (FLIM)
Objectives:
- XLPLN25XWMP2 25x/1.05 water, FWD 2 mm, CG 0 - 0.23 mm, brain sample
index mismatch correction collar
[FWD = free working distance, CG = cover glass]
Fluorescence excitation sources:
Coherent Chameleon Vision (680 nm and 1040 nm tunable) fs-laser
Detectors and cameras:
Olympus internal detectors:
- 2x multialkali photomultiplier tube
- 2x GaAsP photomultiplier tube
External FLIM detector:
- PMA hybrid 40 mod (PicoQuant)
Emission filters:
For Olympus internal detectors:
- 495-540 nm
- 575-645 nm
For external FLIM detector (only a single filter at a time):
- 458/64
- 482/36
- 535/50
- 580/70
- 600/50
Software:
- FluoView
- SymPhoTime 64
Other features:
- Resonant scanner for fast imaging
- Time-correlated single photon couting (TCSPC) FLIM module (PicoQuant PicoHarp 300)
- Piezo focus drive
- Motorised stage
- Motorised focus
| Usage fees [SGD/hour] | LKCMed | NTU | Academia | Industry |
| 10* | 35 | 66 | 105.6 | |
| Location | CSB level 10, Support room 7 | |||
| Safety Notice | Requires N3 laser license | |||
| Contact | nobic.facilities@e.ntu.edu.sg | |||
*Reduced rates apply:
- off-peak hours (weekends, public holidays and 18.00 - 8.30 on weekdays): 70% of the rate stated in the table
- long-term:
- 30% of the prevailing rate applicable after 10 hours of booking/usage
- 30% of the prevailing rate (that is already the discounted rate in this case) applicable after 24 hours of booking/usage.