Carl Zeiss Axio Scan.Z1 slide scanner allows high-throughput automated imaging of large numbers of slides loaded automatically from a magazine with the capacity of 100 slides. A sensitive sCMOS camera allows detecting weak fluorescence while a colour camera is suitable for transmitted light imaging of histologically stained samples.
Available techniques:
- Transmitted light imaging
- Widefield multi-channel fluorescence imaging
- Automated scanning of large numbers of slides
Objectives:
- Plan Apochromat 5x/0.16 dry, FWD 12.1 mm, CG 0.17 mm
- Fluar 5x/0.25 dry, FWD 12.5 mm, CG 0.17 mm
- Plan Apochromat 10x/0.45 dry, FWD 2.0 mm, CG 0.17 mm
- Plan Apochromat 20x/0.8 dry, FWD 0.55 mm, CG 0.17 mm
- Plan Apochromat 40x/0.95 dry, FWD 0.25 mm, CG 0.13 - 0.21 mm
[FWD = free working distance, CG = cover glass]
Fluorescence excitation sources:
- Colibri 7 LED lamp (excitation filters: 385/30; 423/44; 469/38; 555/30; 590/27; 631/33; 735/40)
Filter sets:
Dichroic beamsplitters:
- 405 + 493 + 575 + 654 + 761
- 450 + 538 + 610
- 395
- 495
- 570
Emission filters:
- Multi-band (425/30 + 514/30 + 592/25 + 681/45 + 785/38)
- Triple-band (467/24 + 555/25 + 687/145)
- DAPI (445/50)
- GFP (525/50)
- Cy 5 (690/50)
Detectors and cameras:
- Hamamatsu Orca Flash 4.0 V2 sCMOS (2048x2048 pixels, 6.5 µm/pixel)
- Hitachi HV-F202SCL 3 CCD progressive scan colour camera (1688x1248 pixels, 4.4 µm/pixel)
Software:
- ZEN Blue
Other features:
- automatic loading of slides from a magazine of 100 slides (76 x 26 mm)
- a colour camera for quick slide preview
- barcode recognition (for slide labels)
| Usage fees [SGD/hour] | LKCMed | NTU | Others (Academia/Industry) |
| 5* | 20 | 30 / 48 | |
| Location | CSB 11-02T-01 | ||
| Contact | nobic.facilities@e.ntu.edu.sg | ||
*Reduced rates apply:
- off-peak hours (weekends, public holidays and 18.00 - 8.30 on weekdays): 70% of the rate stated in the table
- long-term:
- 30% of the prevailing rate applicable after 10 hours of booking/usage
- 30% of the prevailing rate (that is already the discounted rate in this case) applicable after 24 hours of booking/usage.