Perkin-Elmer Operetta CLS is a high throughput confocal (spinning disk) and widefield fluorescence microscope with sensitive sCMOS camera, fluorescence filters and 4 LED light sources catering to the most common fluorophores across the whole visible spectrum. Sample environment control (temperature, carbon dioxide and humidity) and automated immersion water delivery to water immersion objectives allow long term live-cell experiments. Hardware autofocus ensures stable focusing throughout long-term experiments.
Available techniques:
- Transmitted light imaging (brightfield, computational phase contrast - constructed 2 images acquired at different axial position)
- Widefield multi-channel fluorescence imaging with a fast and sensitive camera
- Confocal (spinning disk) imaging
- Long-term time-lapse imaging of living cells (with environment control and hardware autofocus)
- Fast multi-well plate screening
Objectives:
- Plan-Apochromat 5x/0.16 dry, FWD 12.1 mm, CG 0.17 mm
- EC Plan-Neofluar 10x/0.3 dry, FWD 5.2 mm, CG 0.17 mm
- LD Plan-Neofluar 20x/0.4 dry, FWD 8.4 - 7.4 mm, CG 0 - 1.5 mm
- W Plan-Apochromat 20x/1.0 water, FWD 1.7 mm, CG 0.17 mm
- LD C-Apochromat 40x/1.1 water, FWD 0.62 mm, CG 0.14 - 0.19 mm
- LD C-Apochromat 63x/1.15 water, FWD 0.6 mm, CG 0.14 - 0.19 mm
[FWD = working distance, CG = cover glass]
Fluorescence excitation sources:
- LEDs:
- 355-385 nm
- 390-420 nm
- 435-460 nm
- 460-490 nm
- 490-515 nm
- 530-560 nm
- 615-645 nm
- 650-675 nm
Transmitted light source:
- IR LED (740 nm) for low phototoxicity
Filter sets:
Emission filters:
- DAPI (430-500 nm)
- CFP (470-515 nm)
- GFP (500-550 nm)
- YFP (525-580 nm)
- dsRed (570-650 nm)
- DRAQ5 (655-760 nm)
Detectors and cameras:
- sCMOS monochrome camera (2160x2160 pixels, 6.5 µm/pixel)
Software:
- Harmony 4.9
Other features:
- Motorised stage
- Motorised focus drive
- Motorised filter turret
- Temperature (to 42°C) and CO2 concentration control
- Hardware autofocus
| Usage fees [SGD/hour]: | LKCMed | NTU | Others (Academia/Industry) |
| Widefield | 7.5* | 20 | 30 / 48 |
| Spinning disk | 45 / 72 | ||
| Location | CSB 11-02T-01 | ||
| Contact | nobic.facilities@e.ntu.edu.sg | ||
*Reduced rates apply:
- off-peak hours (weekends, public holidays and 18.00 - 8.30 on weekdays): 70% of the rate stated in the table
- long-term:
- 30% of the prevailing rate applicable after 10 hours of booking/usage
- 30% of the prevailing rate (that is already the discounted rate in this case) applicable after 24 hours of booking/usage.