Nikon AX R is a laser scanning confocal microscope equipped with a resonant scanner for fast confocal imaging, laser and detector for NIR fluorescence imaging, spectrally freely selectable detection channels and NSPARC detector for super-resolution imaging. The microscope is housed in an enclosure with temperature, CO2 and humidity control for long-term live-cell imaging. Hardware autofocus ensures stable focusing during long-term imaging.
Available techniques:
- 3-dimensional confocal imaging (fast with resonant scanner or high resolution with galvano scanner)
- Super-resolution imaging with NSPARC detector
- NIR fluorescence imaging (730 nm excitation)
- Long-term time-lapse imaging of living cells (with hardware autofocus and environment control)
Objectives:
Note that only 6 objective lenses can be installed at a time; for currently installed objectives refer to the booking system. You may request different objectives when booking the microscope.
- CFI Plan Apochromat Lambda D 2x/0.1 dry, FWD 8.5 mm, CG 0-0.17 mm
- CFI Plan Apochromat Lambda D 10x/0.45 dry, FWD 4.0 mm, CG 0.17 mm (DIC)
- CFI Plan Apochromat Lambda D 20x/0.8 dry, FWD 0.8 mm, CG 0.17 mm (DIC)
- CFI Apo LWD λS 20x/0.95 water immersion, FWD 0.9-0.99 mm, CG 0.11-0.23 mm
- CFI Plan Apochromat Lambda S 25x/1.05 silicone, FWD 0.55 mm, CG 0.11-0.23 mm
- CFI Plan Apochromat Lambda D 40x/0.95 dry, FWD 0.21 mm, CG 0.17 mm (DIC)
- CFI Apo LWD λS 40x/1.15 water immersion, FWD 0.59-0.61 mm, CG 0.15-0.19 mm (DIC)
- CFI Plan Apochromat Lambda S 40x/1.25 silicone, FWD 0.3 mm, CG 0.13-0.21 mm
- CFI Plan Apochromat Lambda D 60x/1.42 oil, FWD 0.15 mm, CG
0.17 mm (DIC)
[FWD = free working distance, CG = cover glass]
Excitation laser lines:
- 405 nm
- 445 nm
- 488 nm
- 561 nm
- 640 nm
- 730 nm
Dichroics and beamplitters:
- 405/488/561/640
- 405/488/561/640/730
- 445/514/590/730
Detectors and cameras:
- 2x GaAsP photomultipliers
- 2x multialkali photomultipliers
- Ex Red GaAsP photomultiplier (NIR optimised)
- NSPARC super-resolution detector (Si single-pixel photon counter array)
- Trasmitted light photomultiplier
Software:
- NIS Elements
Other features:
- Heated enclosure with CO2 atmosphere control
- Automatic immersion water dispenser (for long-term acquisition with water immersion objectives)
- Motorised stage
- Motorised focus drive
- Perfect focus hardware autofocus
| Usage fees [SGD/hour]: | LKCMed | NTU | Others (Academia/Industry) |
| Confocal | 25* | 50 | 60 / 96 |
| Airyscan | |||
| Location | CSB 11-02P-01 (level 11, support room 10) | ||
| Safety Notice | Requires N3 laser license | ||
| Contact | nobic.facilities@e.ntu.edu.sg | ||
*Reduced rates apply:
- off-peak hours (weekends, public holidays and 18.00 - 8.30 on weekdays): 70% of the rate stated in the table
- long-term:
- 30% of the prevailing rate applicable after 10 hours of booking/usage
- 30% of the prevailing rate (that is already the discounted rate in this case) applicable after 24 hours of booking/usage.