Carl Zeiss LSM 800 is a laser scanning confocal microscope, which allows simultaneous imaging in two spectrally freely selectable detection channels. It is equipped with water dipping objectives suitable for example for zebrafish embryos imaging.
Available techniques:
- 3-dimensional confocal imaging
Objectives:
- EC Plan-Neofluar 5x/0.15 dry, FWD 13.6 mm, CG 0.17 mm
- EC Plan-Neofluar 10x/0.3 dry, FWD 5.2 mm, CG 0.17 mm
- W N-Achroplan 20x/0.5 water dipping, FWD 2.6 mm, CG -
- W Plan-Apochromat 40x/1.0 water dipping, FWD 2.5 mm, CG -
- W Plan-Apochromat 63x/1.0 water dipping, FWD 2.1 mm, CG -
[FWD = free working distance, CG = cover glass]
Excitation laser lines:
- 405 nm
- 488 nm
- 561 nm
- 640 nm
Dichroics and beamplitters:
- MBS 405/488/555/639
Detectors and cameras:
- 2x Multi-alkali photomultipliers
- Trasmitted light PMT
Software:
- ZEN Blue
Other features:
- Motorised stage
- Motorised focus drive
| Usage fees [SGD/hour] | LKCMed | NTU | Others (Academia/Industry) |
| 10* | 35 | 45 / 72 | |
| Location | EMB 04-02Y, Zebrafish facility (apply for card access) | ||
| Safety Notice | Requires N3 laser license (N2/04966/0066) or signing N3 waiver SOP | ||
| Contact | nobic.facilities@e.ntu.edu.sg | ||
*Reduced rates apply:
- off-peak hours (weekends, public holidays and 18.00 - 8.30 on weekdays): 70% of the rate stated in the table
- long-term:
- 30% of the prevailing rate applicable after 10 hours of booking/usage
- 30% of the prevailing rate (that is already the discounted rate in this case) applicable after 24 hours of booking/usage.