Carl Zeiss LSM 800 is a laser scanning confocal microscope equipped with two highly sensitive GaAsP PMTs for two spectrally freely selectable detection channels and a GaAsP Airy scan detector for super-resolution imaging. The microscope is housed in an enclosure with temperature, CO₂ and humidity control for long-term live-cell imaging. Hardware autofocus ensures stable focusing during long-term imaging.

Available techniques:

3-dimensional confocal imaging
Super-resolution imaging with Airyscan detector (1.7 x improved resolution in all directions)
Long-term time-lapse imaging of living cells (with hardware autofocus and environment control)

Objectives:

Plan Apochromat 10x/0.45 dry, FWD 2.0 mm, CG 0.17 mm (DIC)
Plan Apochromat 20x/0.8 dry, FWD 0.55 mm, CG 0.17 mm (DIC)
LD-Plan Neofluar 40x/0.6 dry, FWD 3.3 - 2.5 mm, CG 0 - 1.5 mm (DIC)
Plan Apochromat 40x/1.3 oil, FWD 0.21 mm, CG 0.17 mm (DIC)
C-Apochromat 63x/1.2 water, FWD 0.28 mm (for 0.17 mm CG), CG 0.14-0.19 mm (DIC)
Plan Apochromat 63x/1.4 oil, FWD 0.19 mm, CG 0.17 mm (DIC)
[FWD = free working distance, CG = cover glass]

Excitation laser lines:

405 nm
488 nm
561 nm
640 nm

Dichroics and beamplitters:

MBS 405/488/555/639

Detectors and cameras:

2x GaAsP photomultipliers
Airyscan GaAsP super-reolution detector, compatible with 63x objective
Trasmitted light PMT

Software:

ZEN Blue

Other features:

Heated enclosure with CO₂ atmosphere control
Motorised stage
Motorised focus drive
Definite Focus.2 hardware autofocus

Usage fees [SGD/hour]:	LKCMed	NTU	Others (Academia/Industry)
Confocal	12.5*	40	45 / 72
Airyscan			60 / 96
Location	CSB 11-02T-01
Safety Notice	Requires N3 laser license (N2/04966/0067) or signing N3 waiver SOP
Contact	nobic.facilities@e.ntu.edu.sg

*Reduced rates apply:

off-peak hours (weekends, public holidays and 18.00 - 8.30 on weekdays): 70% of the rate stated in the table

long-term:

30% of the prevailing rate applicable after 10 hours of booking/usage

30% of the prevailing rate (that is already the discounted rate in this case) applicable after 24 hours of booking/usage.