Carl Zeiss LSM 800 is a laser scanning confocal microscope equipped with two highly sensitive GaAsP PMTs for two spectrally freely selectable detection channels and a GaAsP Airy scan detector for super-resolution imaging.
Available techniques:
- 3-dimensional confocal imaging
- Super-resolution imaging with Airyscan detector (1.7 x improved resolution in all directions)
Objectives:
- EC Plan-Neofluar 2.5x/0.085 dry, FWD 8.8 mm, CG 0.17 mm
- EC Plan-Neofluar 10x/0.3 dry, FWD 5.2 mm, CG 0.17 mm
- Plan Apochromat 20x/0.8 dry, FWD 0.55 mm, CG 0.17 mm (DIC)
- Plan Apochromat 40x/1.3 oil, FWD 0.21 mm, CG 0.17 mm (DIC)
- Plan Apochromat 63x/1.4 oil, FWD 0.19 mm, CG 0.17 mm (DIC)
- C-Apochromat 63x/1.2 water, FWD 0.28 mm (for 0.17 mm CG), CG
0.14-0.19 mm (DIC)
[FWD = free working distance, CG = cover glass]
Excitation laser lines:
- 405 nm
- 488 nm
- 561 nm
- 640 nm
Dichroics and beamplitters:
- MBS 405/488/555/639
Detectors and cameras:
- 2x GaAsP photomultipliers
- Airyscan GaAsP super-reolution detector, compatible with 63x objective
- Trasmitted light PMT
Software:
- ZEN Blue
Other features:
- Motorised stage
- Motorised focus drive
| Usage fees [SGD/hour]: | LKCMed | NTU | Others (Academia/Industry) |
| Confocal | 12.5* | 40 | 45 / 72 |
| Airyscan | 60 / 96 | ||
| Location | EMB 07-07-01, Imaging room (apply for card access) | ||
| Safety Notice | Requires N3 laser license (N2/04966/0066) or signing N3 waiver SOP | ||
| Contact | nobic.facilities@e.ntu.edu.sg | ||
*Reduced rates apply:
- off-peak hours (weekends, public holidays and 18.00 - 8.30 on weekdays): 70% of the rate stated in the table
- long-term:
- 30% of the prevailing rate applicable after 10 hours of booking/usage
- 30% of the prevailing rate (that is already the discounted rate in this case) applicable after 24 hours of booking/usage.